PMC

Feedback inhibition of NCgl0950 and NCgl2098 DAHP synthases from C. glutamicum RES167 by aromatic amino acids (Phe, Tyr, and Trp). Symbols: ♦, NCgl0950 DAHP synthase inhibited by Phe; ▪, NCgl0950 DAHP synthase inhibited by Tyr; ▴, NCgl0950 DAHP synthase inhibited by Trp; ⋄, NCgl2098 DAHP synthase inhibited by Phe; □, NCgl2098 DAHP synthase inhibited by Tyr; ▵, NCgl2098 DAHP synthase inhibited by Trp. DAHP synthase activities without the addition of aromatic amino acids were determined to be 0.0081 ± 0.0012 and 0.089 ± 0.009 μmol mg⁻¹ min⁻¹, respectively. These average activities were calculated as 100%. Data are averages of the results from three parallel determinations.

The production of PCN in different DAHP synthase overexpression strains.

The expression differences of different DAHP synthase enzymes by transcriptome and relative quantitative PCR analysis. (A) Based on transcriptome analysis; (B) Based on relative quantitative PCR analysis.

Transcriptional level of different DAHP synthase genes and inhibition of PCN on phzC and phzE transcription. (A) Based on absolute quantification; (B) Based on relative quantification. Calculation of the copy numbers of target gene was shown in .